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  • High-efficiency selection of aptamers for bovine

    01-12-2019· In the selection process, ssCE demonstrated high-efficiency selection with bulk Kd reaching at 0.19 ± 0.04 μM by only two rounds, as compared to capillary zone electrophoresis (CZE) mode with Kd of 0.39 ± 0.03 μM.

  • High efficiency selection of full-length cDNA by improved

    Consequently, mRNA remains protected from chemical and enzymatic degradation during the overnight biotinylation step, thus making it possible to select full-length cDNAs of longer average size. We herein report the efficiency and specificity of the new version of the protocol for cap structure biotinylation and capture of full-length cDNA.

  • High-efficiency selection of aptamers for bovine

    01-12-2019· In this study, ssCE presented a high-efficiency selection with enrichment of the best sub-library, similar to CZE by only two rounds. Aptamers were selected for bovine lactoferrin (BLF) protein, a non-heme iron-binding glycoprotein having prime importance in infants metabolism [

  • High-Efficiency, Selection-free Gene Repair in Airway Stem

    High-Efficiency, Selection-free Gene Repair in Airway Stem Cells from Cystic Fibrosis Patients Rescues CFTR Function in Differentiated Epithelia Cystic fibrosis (CF) is a monogenic disorder caused by mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. Mortality in CF patients is mostly due to respiratory sequelae.

  • (PDF) High Efficiency Selection of Full-length cDNA by

    High Efficiency Selection of Full-length cDNA by Improved Biotinylated We herein report the efficiency and specificity of the new version of the protocol for cap structure biotinylation and

  • Evolution of multi-functional capillary electrophoresis

    01-12-2019· Efficient selection: CE's efficiency displays mainly on its high-resolution, thus achieving the efficiency values of 10 4 –10 5 with the improvement at least one orders of magnitude, whereas the upper limit of separation efficiency by chromatographic support or filter is 10 3 (Berezovski et al., 2005; Tok et al., 2010).

  • High-Efficiency, Selection-free Gene Repair in Airway Stem

    06-02-2020· Average CFTR function is approximately 60% higher in corrected HBECs (50% of non-CF levels) than UABCs (30% of non-CF levels). However, since the UABCs and HBECs were not from the same donors, it is unclear whether the differences are due to underlying biology of these related yet distinct airway basal cell populations or due to the distinct genetic backgrounds of the individual donors.

  • Undesirable side effects of selection for high production

    01-10-1998· Animals in a population that have been genetically selected for high production efficiency seem to be more at risk for behavioural, physiological and immunological problems. This paper presents examples of undesirable (cor)related effects of selection for high production efficiency in

  • High-efficiency transfection and drug selection of

    29-06-2006· High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei Chris J Janse 1,Jai Ramesar 1 &

  • High Efficiency Speakers Troels Gravesen

    Aiming at really, really high-efficiency, there are prices to be paid like in any extreme direction. 2-3 watts from a 2A3 SET triode is not a whole lot of power. Some manufacturers claim their 2 watt SET amps to perform well with 90 dB (minimum) speakers. Yes, yes, if you never ever play at

  • High efficiency selection of full-length cDNA by improved

    High efficiency selection of full-length cDNA by improved biotinylated cap trapper DNA Res. 1997 Feb 28;4(1):61-6. doi: 10.1093/dnares/4.1.61. Authors P Carninci 1,A Westover, Y Nishiyama, T Ohsumi, M Itoh, S Nagaoka, N Sasaki, Y Okazaki, M Muramatsu, C

  • High Efficiency Selection of Full-length cDNA by

    High Efficiency Selection of Full-length cDNA by Improved Biotinylated Cap Trapper * Piero Carninci, Piero Carninci 1. Genome Science Laboratory, Tsukuba Life Science Centre, The Institute of Physical and Chemical Research (RIKEN) 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan. 2.

  • How to Develop and Prove High-Efficiency Selection of

    07-04-2021· Request PDF How to Develop and Prove High-Efficiency Selection of Ligands from Oligonucleotide Libraries: A Universal Framework for Aptamers and DNA-Encoded Small-Molecule Ligands Screening

  • High Efficiency Selection Of Stable Cell Lines Selectable

    High Efficiency Selection Of Stable Cell Lines Selectable Markers. Last Updated on Wed, 16 Dec 2020 Mammalian Cells. In order to differentiate cells that have integrated the plasmid into the host genome, a selectable marker that allows identification and selection of those cells is required.

  • (PDF) High Efficiency Selection of Full-length cDNA by

    High Efficiency Selection of Full-length cDNA by Improved Biotinylated We herein report the efficiency and specificity of the new version of the protocol for cap structure biotinylation and

  • High-efficiency transfection and drug selection of

    29-06-2006· High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei Chris J Janse 1,Jai Ramesar 1 &

  • High-efficiency phosphinothricin-based selection for

    Despite the significant advantages of using herbicide resistance for selection of genetically engineered plants, alfalfa transformation has relied primarily on selection for antibiotic resistance. In the few studies reporting the use of resistance to the herbicide phosphinothricin (PPT), transformation efficiencies were low. The present investigation describes a PPT-based selection system for

  • Undesirable side effects of selection for high production

    01-10-1998· 1.. IntroductionGenetic selection has increased production levels of livestock species considerably. Since feed costs are economically the most important costs, the breeding goal in most livestock species is to create a population with high economic production efficiency, i.e. high production combined with relatively low feed intake (Luiting, 1990).

  • NIOSH Guide to the Selection & Use of Particulate

    Selection of filter efficiency (i.e., 95%, 99%, or 99.97%) depends on how much filter leakage can be accepted. Higher filter efficiency means lower filter leakage. The choice of facepiece depends on the level of protection needed–that is, the assigned protection factor (APF) needed. *Code of Federal Regulations. See CFR in references.

  • PREMIUM EFFICIENCY MOTOR SELECTION AND APPLICATION

    PREMIUM EFFICIENCY MOTOR SELECTION AND APPLICATION GUIDE i ACKNOWLEDGMENTS The Premium Efficiency Motor Selection and Application Guide and its companion publication, Continuous Energy Improvement in Motor-Driven Systems, have been developed by the U.S. Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy (EERE) with support from the

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